A Narrative Review of the Putative Etiologic Role and Diagnostic Utility of the Cervicovaginal Microbiome in Human Papillomavirus-Associated Cervical Carcinogenesis

J Med Virol. 2024 Nov;96(11):e70027. doi: 10.1002/jmv.70027.

Abstract

The cervicovaginal microbiome (CVM) may contribute to human papillomavirus (HPV)-associated cervical carcinogenesis. We summarized the literature on the CVM in cervical carcinogenesis by searching Medline, Web of Science, and Embase for articles that sequenced the CVM using metagenomics. Additionally, we identified studies assessing the diagnostic role of the CVM in cervical carcinogenesis by searching PubMed. We performed an environmental scan of Google and Google Scholar to review common CVM characterization techniques. Twenty-eight records presented or summarized associations between the CVM and HPV acquisition, prevalence, persistence, clearance, and cervical lesions or cancer, while three studies identified bacterial taxa detecting high-risk HPV prevalence or cervical lesions. The area under the curve ranged from 0.802 to 0.952. 16S ribosomal RNA gene sequencing and whole metagenome sequencing have sufficient resolution to study the CVM bacteriome. Bacterial communities may have important implications in cervical cancer; however, there is a need for methodological standardization for CVM characterization.

Keywords: cervical cancer; cervicovaginal microbiome; human papillomavirus.

Publication types

  • Review

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Carcinogenesis
  • Cervix Uteri* / microbiology
  • Cervix Uteri* / virology
  • Female
  • Human Papillomavirus Viruses
  • Humans
  • Metagenomics / methods
  • Microbiota*
  • Papillomaviridae* / classification
  • Papillomaviridae* / genetics
  • Papillomaviridae* / isolation & purification
  • Papillomavirus Infections* / diagnosis
  • Papillomavirus Infections* / microbiology
  • Papillomavirus Infections* / virology
  • RNA, Ribosomal, 16S / genetics
  • Uterine Cervical Neoplasms* / diagnosis
  • Uterine Cervical Neoplasms* / microbiology
  • Uterine Cervical Neoplasms* / virology
  • Vagina* / microbiology
  • Vagina* / virology

Substances

  • RNA, Ribosomal, 16S