Novel time-resolved fluorescent-based multiplex immunochromatography test strip for simultaneous detection of pesticides in vegetables

Zhengtao Li; Haowei Dong; Haifang Wang; Donghan Li; Shuxian Zhou; Shengxi Zhai; Jingcheng Huang; Rui Xu; Wenping Zhao; Mohamed Bedair M Ahmed; Gili Bisker; Xia Sun; Yemin Guo

doi:10.1016/j.foodchem.2024.141916

Novel time-resolved fluorescent-based multiplex immunochromatography test strip for simultaneous detection of pesticides in vegetables

Food Chem. 2025 Feb 1;464(Pt 3):141916. doi: 10.1016/j.foodchem.2024.141916. Epub 2024 Nov 5.

Authors

Zhengtao Li¹, Haowei Dong¹, Haifang Wang², Donghan Li¹, Shuxian Zhou¹, Shengxi Zhai¹, Jingcheng Huang¹, Rui Xu¹, Wenping Zhao³, Mohamed Bedair M Ahmed⁴, Gili Bisker⁵, Xia Sun⁶, Yemin Guo⁷

Affiliations

¹ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
² Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.
³ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
⁴ Food Toxicology and Contaminants Dept., National Research Centre, 33 El-Bohouth St., Dokki, Cairo 12622, Egypt.
⁵ Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 6997801, Israel.
⁶ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: [email protected].
⁷ School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: [email protected].

PMID: 39522382
DOI: 10.1016/j.foodchem.2024.141916

Abstract

The presence of multiple pesticide residues in vegetables has become an increasingly critical concern. Immunochromatography has garnered significant interest for its convenience, sensitivity and speed. However, conventional immunochromatography was typically limited to single-target detection, exhibiting restricted efficiency and high costs in on-site large-scale detection scenarios. Dual-channel time-resolved fluorescent immunochromatography test strip (TRFITS) was developed for the simultaneous rapid detection of imidacloprid (IMI) and tolfenpyrad (TOL) in 15 min. Utilizing the excellent optical properties of time-resolved fluorescent microspheres, this TRFITS enabled better detection sensitivity (0.22 ng/mL for IMI, 11.85 ng/mL for TOL), which was approximately 20 times more sensitive than that of conventional immunochromatography, under optimal conditions. In addition, the TRFITS successfully detected IMI and TOL in vegetables samples with acceptable accuracy. The ability to simultaneously quantify multiple pesticide residues using a single test strip reduces material consumption and lowers operational costs, offering a practical solution for rapid on-site pesticide screening.

Keywords: Imidacloprid; Simultaneous detection; Time-resolved fluorescent immunochromatography test strip; Tolfenpyrad; Vegetable.

Publication types

Evaluation Study

MeSH terms

Chromatography, Affinity* / instrumentation
Chromatography, Affinity* / methods
Fluorescence
Food Contamination* / analysis
Neonicotinoids / analysis
Nitro Compounds
Pesticide Residues* / analysis
Pesticides / analysis
Reagent Strips / analysis
Vegetables* / chemistry

Substances

Pesticide Residues
Reagent Strips
Neonicotinoids
Pesticides
imidacloprid
Nitro Compounds