Enhanced CO2 Coordinates the Spatial Recruitment of Diazotrophs in Rice Via Root Development

Junwen Zhao; Yuting Chen; Qi Tao; Lukas Schreiber; Kiran Suresh; Michael Frei; Muhammad Shahedul Alam; Bing Li; Yaping Zhou; Marcel Baer; Frank Hochholdinger; Changquan Wang; Peng Yu

doi:10.1111/pce.15259

Enhanced CO₂ Coordinates the Spatial Recruitment of Diazotrophs in Rice Via Root Development

Plant Cell Environ. 2024 Nov 11. doi: 10.1111/pce.15259. Online ahead of print.

Authors

Junwen Zhao^{1

2

3

4}, Yuting Chen¹, Qi Tao¹, Lukas Schreiber⁵, Kiran Suresh⁵, Michael Frei⁶, Muhammad Shahedul Alam⁶, Bing Li¹, Yaping Zhou³, Marcel Baer^{2

3}, Frank Hochholdinger³, Changquan Wang¹, Peng Yu^{2

3

7}

Affiliations

¹ College of Resources, Sichuan Agricultural University, Chengdu, China.
² Emmy Noether Group Root Functional Biology, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany.
³ Crop Functional Genomics, Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany.
⁴ Institute of Agricultural Engineering, Chongqing Academy of Agricultural Sciences, Chongqing, China.
⁵ Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany.
⁶ Department of Agronomy and Crop Physiology, Institute for Agronomy and Plant Breeding I, Justus Liebig University Giessen, Giessen, Germany.
⁷ Plant Genetics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany.

PMID: 39526402
DOI: 10.1111/pce.15259

Abstract

Understanding the reciprocal interaction between root development and coadapted beneficial microbes in response to elevated CO₂ (eCO₂) will facilitate the identification of nutrient-efficient cultivars for sustainable agriculture. Here, systematic morphological, anatomical, chemical and gene expression assays performed under low-nitrogen conditions revealed that eCO₂ drove the development of the endodermal barrier with respect to L-/S-shaped lateral roots (LRs) in rice. Next, we applied metabolome and endodermal-cell-specific RNA sequencing and showed that rice adapts to eCO₂ by spatially recruiting diazotrophs via flavonoid secretion in L-shaped LRs. Using the rice Casparian strip mutant Oscasp1-1, we confirmed that reduced lignin deposition selectively recruits the diazotrophic family of Oxalobacteraceae to confer tolerance to low nitrogen availability.

Keywords: beneficial microbes; elevated CO2; nutrient efficiency; root development.

Abstract

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