Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum

Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain 'XM-1' with dynamic color changes of Oncidium were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor Cluster-100966.1_OnDIVARICATA 3 was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and Arabidopsis thaliana was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing OnDIVARICATA 3 transgenic hairy roots, the expression of flower color related genes FLS, ZEP, and CHYB were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of Oncidium hybridum, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of Oncidium hybridum.