In narrative identity research, variables are typically captured through detailed content-coding of personal narratives. Yet, alternative methods have been suggested, notably self-report scales, because they capture a participant's own interpretation of their personal narratives, and because they are efficient to administer as a supplement to more labor-intensive coding methods. This study developed and validated the Narrative Identity Self-Evaluation (NISE) questionnaire. In Study 1, the questionnaire was developed through exploratory factor analysis (n = 425) and its criterion validity examined. In Study 2, the NISE factor structure and criterion relationships were confirmed (n = 304). In Study 3 (based on the same sample as Study 1), content-coding of 11 narrative identity characteristics in open-ended personal story accounts was conducted, and NISE scores were compared to corresponding content-coded variables. The 20-item NISE has three factors replicating common dimensions in narrative identity (autobiographical reasoning, desire for structure, positive motivational/affective themes) and a novel fourth factor capturing disturbances of narrative identity. The NISE correlated in theoretically-coherent ways with content coded narrative identity variables, self-report traits, and measures relevant for narrative identity, self-concept, well-being, and psychopathology. We discuss the scale's advantages in complementing content-coding of narrative accounts to assess variation in narrative identity within both clinical and non-clinical populations.