Dietary zinc deficiency promotes Acinetobacter baumannii lung infection via IL-13 in mice

Nat Microbiol. 2024 Dec;9(12):3196-3209. doi: 10.1038/s41564-024-01849-w. Epub 2024 Nov 15.

Abstract

Dietary zinc deficiency is a major risk factor for pneumonia. Acinetobacter baumannii is a leading cause of ventilator-associated pneumonia and a critical public health threat due to increasing rates of multidrug resistance. Patient populations at increased risk for A. baumannii pneumonia are also at increased risk of zinc deficiency. Here we established a mouse model of dietary zinc deficiency and acute A. baumannii pneumonia to test the hypothesis that host zinc deficiency contributes to A. baumannii pathogenesis. We showed that zinc-deficient mice have significantly increased A. baumannii burdens in the lungs, dissemination to the spleen and higher mortality. During infection, zinc-deficient mice produce more pro-inflammatory cytokines, including IL-13. Administration of IL-13 promotes A. baumannii dissemination in zinc-sufficient mice, while antibody neutralization of IL-13 protects zinc-deficient mice from A. baumannii dissemination and mortality during infection. These data highlight the therapeutic potential of anti-IL-13 antibody treatments, which are well tolerated in humans, for the treatment of pneumonia.

MeSH terms

  • Acinetobacter Infections* / immunology
  • Acinetobacter Infections* / microbiology
  • Acinetobacter baumannii* / immunology
  • Animals
  • Cytokines / metabolism
  • Diet
  • Disease Models, Animal*
  • Female
  • Humans
  • Interleukin-13* / deficiency
  • Interleukin-13* / genetics
  • Interleukin-13* / metabolism
  • Lung* / immunology
  • Lung* / microbiology
  • Lung* / pathology
  • Mice
  • Mice, Inbred C57BL
  • Pneumonia, Bacterial / immunology
  • Pneumonia, Bacterial / microbiology
  • Spleen / immunology
  • Spleen / microbiology
  • Zinc* / administration & dosage
  • Zinc* / deficiency
  • Zinc* / metabolism

Substances

  • Interleukin-13
  • Zinc
  • Cytokines