Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

Alba Orea-Soufi; David Dávila; María Salazar-Roa; Mar Lorente; Guillermo Velasco

doi:10.1007/978-1-0716-4217-7_2

Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

Methods Mol Biol. 2025:2871:11-18. doi: 10.1007/978-1-0716-4217-7_2.

Authors

Alba Orea-Soufi^{1

2}, David Dávila^{3

4}, María Salazar-Roa^{3

4}, Mar Lorente^{3

4}, Guillermo Velasco^{5

6}

Affiliations

¹ ABC-RI, Algarve Biomedical Center Research Institute, Algarve Biomedical Center, Faro, Portugal.
² Faculty of Medicine and Biomedical Sciences, University of Algarve, Faro, Portugal.
³ Department of Biochemistry and Molecular Biology, School of Biology, Complutense University, Madrid, Spain.
⁴ Instituto de Investigaciones Sanitarias San Carlos (IdISSC), Madrid, Spain.
⁵ Department of Biochemistry and Molecular Biology, School of Biology, Complutense University, Madrid, Spain. [email protected].
⁶ Instituto de Investigaciones Sanitarias San Carlos (IdISSC), Madrid, Spain. [email protected].

PMID: 39565574
DOI: 10.1007/978-1-0716-4217-7_2

Abstract

Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.

Keywords: Cell fractioning; FOXO phosphorylation; Immunofluorescence; Western blot.

MeSH terms

Animals
Forkhead Transcription Factors* / metabolism
Humans
Phosphorylation

Substances

Forkhead Transcription Factors