Discovery of two new long chain fatty acid esters of flavonol glycoside from Pollen Typhae Carbonisatus and their protective effects on HBMECs

J Asian Nat Prod Res. 2024 Nov 21:1-10. doi: 10.1080/10286020.2024.2428238. Online ahead of print.

Abstract

ABATRACTTwo new compounds, namely, isorhamnenin-3-O-neohesperidin-6''-linoleic acid ester (1) and isorhamnenin-3-O-neohesperidin-6''-palmitate (2), along with 17 known compounds, were isolated from Pollen Typhae Carbonisatus (PTC). Their structures were elucidated on the basis of one-dimensional (1D)-, two-dimensional (2D)-nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HRESIMS) spectroscopic data analyses. The two new compounds (1 and 2) exhibited a protective effect in a dose-dependent manner and promoted tube generation in the oxygen-glucose deprivation/reoxygenation (OGD/R)-induced human brain microvascular endothelial cells (HBMECs).

Keywords: HBMECs; Pollen Typhae Carbonisatus; long chain fatty acid esters of flavonol glycoside.