Development of efficient and accurate detection methods for antibiotics is crucial for ensuring food safety and safeguarding human health. In this study, a dual-mode sensing platform was constructed by integrating photoelectrochemical (PEC) and surface-enhanced Raman scattering (SERS) techniques for the sensitive detection of kanamycin using an aptamer signal conversion strategy which was based on the C3N4/MXene-gold nanoparticles heterojunction. The sensitive dual-mode sensing platform enabled detection of kanamycin, with linear response ranges of 10-3 to 105 nM for PEC with a detection limit (LOD) of 0.53 pM, and 10-2 to 104 nM for SERS with a LOD of 4.8 pM. Notably, this platform exhibited excellent performance for kanamycin assays in raw milk. Furthermore, combined with various aptamer-targets, it can be easily broadened to detect more low concentration target substances in other complex matrix, showcasing its vast potential for diverse applications.
Keywords: Antibiotics; Kanamycin; Photoelectrochemical; Surface-enhanced Raman scattering.
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