Transcription- and Replication-Competent Virus-like Particle Systems for Marburg Virus

Ellen L Suder; Adam J Hume

doi:10.1007/978-1-0716-4256-6_10

Transcription- and Replication-Competent Virus-like Particle Systems for Marburg Virus

Methods Mol Biol. 2025:2877:141-154. doi: 10.1007/978-1-0716-4256-6_10.

Authors

Ellen L Suder¹, Adam J Hume²

Affiliations

¹ Department of Virology, Immunology & Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.
² Department of Virology, Immunology & Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA. [email protected].

PMID: 39585619
DOI: 10.1007/978-1-0716-4256-6_10

Abstract

Here, we describe the transcription- and replication-competent virus-like particle (trVLP) system for Marburg virus (MARV), which recapitulates transcription and replication of the viral genome in addition to viral particle assembly, egress, and entry. This protocol includes instructions for transfections for producer and acceptor cells and the use of trVLPs for infection.

Keywords: Filoviruses; MARV; Minigenomes; Reverse genetics; VLPs; trVLPs.

MeSH terms

Animals
Cell Line
Genome, Viral
Humans
Marburgvirus* / genetics
Marburgvirus* / physiology
RNA, Viral / genetics
Transcription, Genetic*
Transfection / methods
Virion / genetics
Virus Assembly / genetics
Virus Replication* / genetics

Substances

RNA, Viral