Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein Using a Multiplex Immunoassay Platform

Aquena Ball; Teri Ann S Wong; Axel T Lehrer

doi:10.1007/978-1-0716-4256-6_23

Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein Using a Multiplex Immunoassay Platform

Methods Mol Biol. 2025:2877:345-354. doi: 10.1007/978-1-0716-4256-6_23.

Authors

Aquena Ball¹, Teri Ann S Wong¹, Axel T Lehrer²

Affiliations

¹ Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
² Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA. [email protected].

PMID: 39585632
DOI: 10.1007/978-1-0716-4256-6_23

Abstract

Multiplex immunoassays allow for the simultaneous detection of antibody populations targeting multiple antigens in a very sensitive, high-throughput format. This assay has applications in seroprevalence, serodiagnosis, and vaccine assessment with greater sensitivity and dynamic range than standard ELISAs (enzyme-linked immunosorbent assays). Here, we show in detail the methods for multiplex immunoassays to detect IgG to the Marburg virus glycoprotein.

Keywords: High-throughput serology; Multiplex immunoassay; Serodiagnosis; Seroprevalence; Viral antigen profiling.

MeSH terms

Animals
Antibodies, Viral* / blood
Antibodies, Viral* / immunology
Enzyme-Linked Immunosorbent Assay / methods
Glycoproteins* / immunology
Humans
Immunoassay / methods
Immunoglobulin G / blood
Immunoglobulin G / immunology
Marburg Virus Disease / blood
Marburg Virus Disease / diagnosis
Marburg Virus Disease / immunology
Marburgvirus* / immunology

Substances

Antibodies, Viral
Glycoproteins
Immunoglobulin G