Recent developments in xylooligosaccharides: Sustainable production, characterization, beneficial properties and applications

Xylooligosaccharides (XOS) are functional oligosaccharides with prebiotic activity, offering exceptional nutritional and health benefits and a wide range of applications. The increasing market demand for healthy products has driven interest in XOS, as they are low-dose prebiotics with high added value, fostering developments in their production. In a sustainable production context, XOS can be obtained from low-cost lignocellulosic raw materials, which require adapted pretreatment techniques to produce high-quality XOS with high process efficiency. One- and two-step methodologies are discussed in terms of increasing XOS production while aiming to limit the co-production of toxic compounds. Autohydrolysis and enzymatic hydrolysis with xylanases stand out as a promising technology for XOS production with high yields. Purification and characterization methodologies are also two important steps to achieve mixtures of XOS with high purity, specific degree of polymerization and substitution to improve their beneficial properties and, consequently, their applications. This review presents the potential of XOS as prebiotics, describing the recent technological production routes, along with advancements in the development of new production processes using alternative substrates, as well as the beneficial effects of their consumption, addressing their latest applications that justify the pursuit of their consolidation in the industrial prebiotic market.