Genomic analysis and characterization of a new Salmonella phage vB_Sen_ST2 infecting Salmonella enterica serovars Typhi and Typhimurium

Microb Pathog. 2025 Jan:198:107178. doi: 10.1016/j.micpath.2024.107178. Epub 2024 Nov 27.

Abstract

In this study, we have characterized and sequenced the whole genome of a new member of the Kuttervirus genus, Salmonella phage vB_Sen_ST2. This phage selectively targets Salmonella enterica serovars Typhi and Typhimurium, which are major etiologic agents of salmonellosis worldwide. Its genome consists of a linear, double-stranded DNA of 156,028 bp, with a G + C content of 44.93 %. Based on our results, Salmonella phage vB_Sen_ST2 presents suitable features to be considered as a potential control agent against Salmonella enterica serovars that are responsible for salmonellosis.

Keywords: Bacteriophage; Foodborne pathogen; Salmonella; Salmonella phage.

MeSH terms

  • Base Composition*
  • DNA, Viral / genetics
  • Genome, Viral*
  • Genomics / methods
  • Host Specificity
  • Phylogeny
  • Salmonella Phages* / classification
  • Salmonella Phages* / genetics
  • Salmonella Phages* / isolation & purification
  • Salmonella typhi* / genetics
  • Salmonella typhi* / virology
  • Salmonella typhimurium* / genetics
  • Salmonella typhimurium* / virology
  • Sequence Analysis, DNA
  • Whole Genome Sequencing

Substances

  • DNA, Viral