HPTLC detection, extraction - isolation optimization and characterization of in-house isolated Wedelolactone from the whole plant extract of Eclipta prostrata (L.) L. for the development of efficacious formulations

In present study Wedelolactone (WL) was identified from successive extracts of whole plant Eclipta prostrata (L.) L. using HPTLC, optimised extraction/isolation method and confirmed WL structure.Extraction, isolation andidentification was performed though cold-percolation method, column chromatography and TLC respectively.WL was found abundantly in ethyl acetate and ethanol extracts at UV254 & 366 nm, respectively at R_F0.42 by using toluene:ethyl acetate: formic acid (6:3.5:0.5, v/v/v). In the Method IV got a pure pale green crystals (0.138 g, % yield of 0.23) was adopted. Isolated compound verified by TLC and the structure confirmed by various spectroscopic tools. The study proposed high efficiency extraction-isolation method for WL, which could enhance the efficacy for identification of E. prostrata, studying such coumestan in extracts and herbal formulations. Study potentially aiding in developing advance anti-cancer and hepatoprotectivedosages and quality control.