Susceptibility of Gram-negative bacteria from blood cultures assessed by a rapid antimicrobial susceptibility assay, the VITEK® Reveal™: a preliminary study

J Med Microbiol. 2024 Dec;73(12). doi: 10.1099/jmm.0.001941.

Abstract

The timely administration of the effective therapy is an important factor for the favourable outcome of patients with sepsis. In this study, we evaluated a new rapid antimicrobial susceptibility testing (AST) method, the Vitek®Reveal system, to determine the antibiotic susceptibility of 44 Gram-negative bacteria randomly isolated from blood. The results show a mean turnaround time of 5.42 h. The overall agreement with the reference method was >90%, except for piperacillin/tazobactam and cefepime (90.4%). The study, therefore, suggests that the assay decreases the time for obtaining AST with the potential to have a positive impact on patient care. However, further studies are needed to extend and confirm these preliminary findings, particularly the assay performance for some drugs and eventually for Gram-positive micro-organisms.

Keywords: Gram-negative bacteria; antibiotic susceptibility; blood infections; sepsis.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents* / pharmacology
  • Blood Culture* / methods
  • Gram-Negative Bacteria* / drug effects
  • Gram-Negative Bacteria* / isolation & purification
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests* / methods
  • Time Factors

Substances

  • Anti-Bacterial Agents