Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose

Cyun-Ming Wu; Hao-Yun Li; Wen-Yi Li; Chih-Neng Hsu; Wei-Shun Yang; Gwo-Tsang Chuang; Tony Pan-Hou Che; Tung-Yuan Lee; Hsiao-Lin Lee; Siow-Wey Hee; Jiin-Horng Lee; Daniel Liao; Karen Chia-Wen Liao; Ya-Wen Liu; Chin-Feng Hsuan; Yi-Cheng Chang; Shao-Lun Chu

doi:10.1016/j.xpro.2024.103478

Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose

STAR Protoc. 2024 Dec 20;5(4):103478. doi: 10.1016/j.xpro.2024.103478. Epub 2024 Dec 6.

Authors

Cyun-Ming Wu¹, Hao-Yun Li¹, Wen-Yi Li², Chih-Neng Hsu³, Wei-Shun Yang⁴, Gwo-Tsang Chuang⁵, Tony Pan-Hou Che⁶, Tung-Yuan Lee⁶, Hsiao-Lin Lee⁷, Siow-Wey Hee⁸, Jiin-Horng Lee⁹, Daniel Liao⁸, Karen Chia-Wen Liao¹⁰, Ya-Wen Liu¹¹, Chin-Feng Hsuan¹², Yi-Cheng Chang¹³, Shao-Lun Chu¹⁴

Affiliations

¹ Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
² Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
³ Cardiovascular Center, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
⁴ Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, HsinChu Branch, HsinChu 300, Taiwan.
⁵ Division of Nephrology, Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan.
⁶ PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan.
⁷ Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
⁸ Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
⁹ Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
¹⁰ Biological Sciences Division, University of Chicago, Chicago, IL 60637, USA.
¹¹ Institute of Molecular Medicine, National Taiwan University, Taipei 100, Taiwan.
¹² Division of Cardiology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan; Division of Cardiology, Department of Internal Medicine, E-Da Dachang Hospital, I-Shou University, Kaohsiung 807, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan.
¹³ PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taiwan 100, Taiwan. Electronic address: [email protected].
¹⁴ Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan. Electronic address: [email protected].

Abstract

2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.

Keywords: Health Sciences; Metabolism; Metabolomics.

MeSH terms

Animals
Deoxyglucose* / analogs & derivatives
Deoxyglucose* / metabolism
Glucose* / metabolism
Glucose-6-Phosphate / analogs & derivatives
Mice

Substances

Deoxyglucose
Glucose
2-deoxyglucose-6-phosphate
Glucose-6-Phosphate