The impact of adherence counseling incorporating a point of care urine tenofovir assay on virologic suppression among individuals failing tenofovir-lamivudine-dolutegravir: A pre-post intervention study

Int J Infect Dis. 2025 Feb:151:107328. doi: 10.1016/j.ijid.2024.107328. Epub 2024 Dec 6.

Abstract

Objectives: To examine if point-of-care (POC) urine tenofovir testing-informed counseling could be used to improve virologic suppression (VS) among participants with virologic failure (VF) after ≥1 prior round of enhanced adherence counseling (EAC).

Methods: Participants were enrolled from 42 clinics across Namibia. At each monthly medication pick-up, participants completed the POC urine test and received EAC informed by this testing (EAC+). If VS was not achieved after 3 months of EAC+, up to 3 additional rounds of EAC+ were provided, with resistance testing at month (M)9.

Results: Of 310 potentially eligible participants across 42 clinics in Namibia, we enrolled 211 participants with VF (median age 33 years, 61% female); 195 reached M3 defined as receiving EAC+ and follow-up viral load testing; 169 achieved VS within M3 (87%, P < 0.001) and 97% by M9 (181/186) compared to 40% (22/55) prior to the intervention (P < 0.001). Resistance testing was performed in five remaining participants with VF at M9, of whom 1/5 (20%) developed dolutegravir resistance.

Conclusion: The urine tenofovir assay when incorporated into adherence counseling has potential to be a cost-effective intervention among participants failing tenofovir-based regimens, increasing VS to 97% in those failing Tenofovir-Lamivudine-Dolutegravir. Encouraging results of this pre-post intervention will be rigorously tested in a randomized trial.

Keywords: Enhanced adherence counseling; Namibia; People living with HIV; Point-of-care assay; Tenofovir-Lamivudine-Dolutegravir (TLD); Urine tenofovir assay; Virologic suppression.

MeSH terms

  • Adult
  • Anti-HIV Agents* / therapeutic use
  • Counseling*
  • Female
  • HIV Infections* / drug therapy
  • HIV Infections* / urine
  • HIV Infections* / virology
  • HIV-1 / drug effects
  • Heterocyclic Compounds, 3-Ring / therapeutic use
  • Humans
  • Lamivudine* / therapeutic use
  • Male
  • Medication Adherence*
  • Middle Aged
  • Oxazines*
  • Piperazines* / therapeutic use
  • Point-of-Care Systems
  • Pyridones* / therapeutic use
  • Tenofovir* / therapeutic use
  • Treatment Failure
  • Viral Load*

Substances

  • Pyridones
  • Tenofovir
  • Oxazines
  • Anti-HIV Agents
  • Lamivudine
  • dolutegravir
  • Piperazines
  • Heterocyclic Compounds, 3-Ring