Purpose: Colorectal medullary carcinoma is extensive lymphocyte infiltration and associated with an active immune response. However, studies to comprehensively explore the immune landscape and efficacy of immune checkpoint blockade therapy in MeC are limited.
Experimental design: We screened 47 cases of MeC from Harbin Medical University Cancer Hospital cohort. The immunological characteristics of MeC were analyzed by targeted exon sequencing, NanoString nCounter gene expression sequencing, immunohistochemistry, multiplexed immunofluorescence and T cell antigen receptor-sequencing. An additional 47 MeC patients received ICB therapy were included in the retrospective analysis to verify the efficacy of immunotherapy.
Results: Genomically, MeC tend to have a higher proportion of mismatch repair protein deficiency/microsatellite instability, ARID1A mutation, and ASCL2 amplification. Gene expression shows enriched immune response-related pathways while down-regulated oncogenic pathways, such as Glycolysis, Epithelial mesenchymal transition, and Wnt beta catenin signaling. Further immune-characterization showed that MeC showed advantages in antigen presentation, co-stimulatory molecules, effector molecules, immune checkpoints, and immune cell abundance. More importantly, both MSI and microsatellite stable type MeC showed a similar state of high infiltration of immune cells, even better than MSI-nMeC. MeC infiltrated massive highly clonal immune cells, especially intraepithelial CD8+T cells. In the retrospective cohort, there were 30 patients with MeC received ICB achieved complete or partial response with an objective response rate of 63.8%, especially including 16 patients with MSS-CRC.
Conclusion: MeC is a pathological subtype with an active immune response and is a promising group for ICB therapy. This heightened immune response was not limited to the patient's microsatellite status.