A 29-mRNA host response test to identify bacterial and viral infections and to predict 30-day mortality in emergency department patients with suspected infections: A prospective observational cohort study

Kirby Tong-Minh; Leanne van Leeuwen; Christian Ramakers; Uan-I Chen; Oliver Liesenfeld; Diederik Gommers; Eric van Gorp; Henrik Endeman; Yuri van der Does

doi:10.1016/j.diagmicrobio.2024.116599

A 29-mRNA host response test to identify bacterial and viral infections and to predict 30-day mortality in emergency department patients with suspected infections: A prospective observational cohort study

Diagn Microbiol Infect Dis. 2024 Nov 26;111(3):116599. doi: 10.1016/j.diagmicrobio.2024.116599. Online ahead of print.

Authors

Kirby Tong-Minh¹, Leanne van Leeuwen², Christian Ramakers³, Uan-I Chen⁴, Oliver Liesenfeld⁵, Diederik Gommers⁶, Eric van Gorp⁷, Henrik Endeman⁸, Yuri van der Does⁹

Affiliations

¹ Department of Emergency Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands; Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
² Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
³ Department of Clinical Chemistry, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
⁴ Inflammatix Inc., Sunnyvale, California, USA. Electronic address: [email protected].
⁵ Inflammatix Inc., Sunnyvale, California, USA. Electronic address: [email protected].
⁶ Department of Intensive Care, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
⁷ Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands; Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
⁸ Department of Intensive Care, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].
⁹ Department of Emergency Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: [email protected].

PMID: 39657556
DOI: 10.1016/j.diagmicrobio.2024.116599

Abstract

Introduction: The goal of this study is to validate the accuracy of the 29-mRNA host response classifiers Inflammatix Bacterial-Viral-Non infected-3b (IMX-BVN-3b) and Severity-3b (IMX-SEV-3b) to identify bacterial and viral infections and to predict 30-day mortality in patients with suspected infections in the ED.

Methods: This prospective observational cohort study enrolled patients with suspected infections in a tertiary ED. IMX-BVN-3b was compared to clinically forced and consensus adjudicated bacterial/viral infection status and IMX-SEV-3b was compared to 30-day mortality.

Results: A total of 688 patients were enrolled. Using forced adjudication, the AUC for the diagnosis of bacterial infection by IMX-BVN-3b was 0.76 (95 % CI: 0.72 - 0.80). The AUC for the diagnosis of viral infections was 0.89 (95 %CI 0.84-0.95). IMX-SEV-3b had an AUC of 0.77 (95 % CI: 0.68 - 0.85) on 30-day mortality.

Conclusion: The 29-gene host response classifiers IMX-BVN-3b and IMX-SEV-3b identify viral and bacterial infections and predict 30-day mortality in patients with suspected infections in the ED.

Keywords: Bacterial infections; Biomarkers; Emergency department; Transcriptomics; Viral infections.