Macroencapsulation Device with Anti-inflammatory Membrane Modification Enhances Long-Term Viability and Function of Transplanted β Cells

ACS Appl Mater Interfaces. 2024 Dec 25;16(51):70218-70230. doi: 10.1021/acsami.4c14057. Epub 2024 Dec 12.

Abstract

Treating type 1 diabetes (T1D) through β-cell macroencapsulation is a promising long-term solution, but it faces challenges such as immune-mediated fibrosis on the capsule surface, which impairs cell functionality and compromises longevity and effectiveness. This study presents an approach for including an anti-inflammatory molecule on the macroencapsulation device (MED) using initiated chemical vapor deposition for the surface modification of poly(tetrafluoroethylene) (PTFE) membranes. The surface-modified MEDs significantly reduced fibrosis, improved β-cell viability and functionality, and promoted M2 macrophage polarization, which is associated with anti-inflammatory effects. This MED displayed improved glycemic control in a streptozotocin-induced diabetic mouse model for 45 days. The findings underscore the potential of surface-modified MEDs for improving T1D management by mitigating inflammation and enhancing the therapeutic efficacy of β-cell encapsulation.

Keywords: anti-inflammatory molecule; functionalized membrane; initiated chemical vapor deposition (iCVD); macroencapsulation; macrophage polarization; type 1 diabetes (T1D); β cell.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents* / chemistry
  • Anti-Inflammatory Agents* / pharmacology
  • Cell Encapsulation / methods
  • Cell Survival* / drug effects
  • Diabetes Mellitus, Experimental* / pathology
  • Diabetes Mellitus, Type 1 / therapy
  • Insulin-Secreting Cells* / cytology
  • Insulin-Secreting Cells* / drug effects
  • Insulin-Secreting Cells* / metabolism
  • Islets of Langerhans Transplantation
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Membranes, Artificial
  • Mice
  • Mice, Inbred C57BL
  • Polytetrafluoroethylene / chemistry

Substances

  • Anti-Inflammatory Agents
  • Polytetrafluoroethylene
  • Membranes, Artificial