RB-TnSeq barcode abundance data sets for Novosphingobium aromaticivorans grown on the β-5-linked aromatic dimer dehydrodiconiferyl alcohol

Fletcher Metz; Marco N Allemann; Joshua K Michener; Kevin S Myers; Fachuang Lu; Daniel R Noguera; Timothy J Donohue

doi:10.1128/mra.00844-24

RB-TnSeq barcode abundance data sets for Novosphingobium aromaticivorans grown on the β-5-linked aromatic dimer dehydrodiconiferyl alcohol

Microbiol Resour Announc. 2025 Jan 16;14(1):e0084424. doi: 10.1128/mra.00844-24. Epub 2024 Dec 12.

Authors

Fletcher Metz^{1

2

3}, Marco N Allemann⁴, Joshua K Michener⁴, Kevin S Myers^{1

2}, Fachuang Lu^{1

2}, Daniel R Noguera^{1

2

5}, Timothy J Donohue^{1

2

6}

Affiliations

¹ DOE Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, Wisconsin, USA.
² Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, USA.
³ Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin, USA.
⁴ Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
⁵ Department of Civil and Environmental Engineering, University of Wisconsin, Madison, Wisconsin, USA.
⁶ Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, USA.

Abstract

A randomly barcoded transposon insertion sequencing (RB-TnSeq) library of Novosphingobium aromaticivorans DSM12444 was grown in media containing either glucose or the β-5-linked aromatic dimer dehydrodiconiferyl alcohol (DC-A) as the sole carbon source. The cultures were grown to saturation and then sequenced, yielding the barcode abundance data sets presented here.

Keywords: aromatic compounds; genetics; genomics; ligninolysis.

Abstract

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