OsCYCBL1 and OsHTR702 positively regulate rice tolerance to cold stress

Chuan Ma; Shiwei Zheng; Songjin Yang; Jiacheng Wu; Xingzhuo Sun; Yulin Chen; Peng Zhang; Yanting Li; Lingli Wu; Xin Liang; Qiuping Fu; Lihua Li; Jianqing Zhu; Xiaomei Jia; Xiaoying Ye; Zhengjun Xu; Rongjun Chen

doi:10.1016/j.ijbiomac.2024.138642

OsCYCBL1 and OsHTR702 positively regulate rice tolerance to cold stress

Int J Biol Macromol. 2024 Dec 10:287:138642. doi: 10.1016/j.ijbiomac.2024.138642. Online ahead of print.

Authors

Chuan Ma¹, Shiwei Zheng², Songjin Yang¹, Jiacheng Wu¹, Xingzhuo Sun¹, Yulin Chen¹, Peng Zhang¹, Yanting Li¹, Lingli Wu¹, Xin Liang¹, Qiuping Fu¹, Lihua Li¹, Jianqing Zhu³, Xiaomei Jia³, Xiaoying Ye³, Zhengjun Xu¹, Rongjun Chen⁴

Affiliations

¹ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu 611130, China.
² State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu 611130, China. Electronic address: [email protected].
³ Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University 211, Huimin Road, Chengdu 611130, China.
⁴ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu 611130, China; Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University 211, Huimin Road, Chengdu 611130, China; Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province, Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China. Electronic address: [email protected].

PMID: 39667477
DOI: 10.1016/j.ijbiomac.2024.138642

Abstract

Chaling wild rice (Oryza rufipogon Griff.) can survive winter due to its extreme cold tolerance, whereas cultivated rice (Oryza sativa L.) cannot. Here, we found that the expression level of OsCYCBL1 decreased relatively less at low temperatures in Chaling wild rice compared with cultivated rice. Transgenic assays of OsCYCBL1 in Nipponbare (Nip) showed that overexpression of OsCYCBL1 promoted cold tolerance. Transcriptome profiling, RT-qPCR analysis, and physiological parameters measurement indicated that overexpression of OsCYCBL1 maintained better DNA damage repair capacity, balanced the cell cycle, enhanced reactive oxygen species (ROS) homeostasis, and increased wax content, directly affecting the ICE-CBF-COR cascade. Moreover, OsHTR702, a gene that interacts with OsCYCBL1, also positively regulates rice cold tolerance by affecting the ICE-CBF-COR cascade and increasing ROS homeostasis at low temperatures. In addition, overexpression of OsCYCBL1 and OsHTR702 enabled rice to survive through winter. Taken together, the current results indicate that OsCYCBL1 and OsHTR702 are related to cold tolerance in rice, making them potential targets for enhancing crop resilience to cold stress.

Keywords: Cell cycle; Cold tolerance; OsCYCBL1; OsHTR702; Overwinter; ROS; Wild rice.