Simultaneous elimination mechanism of formaldehyde and acrolein by resveratrol in food and the cytotoxicity of the products

Polyphenols have been intensively investigated for scavenging single harmful aldehydes, such as formaldehyde (FA) and acrolein (ACR). However, there is a lack of studies on the effect and mechanism of eliminating co-existing harmful aldehydes by polyphenols. In this study, resveratrol (RV) was found to simultaneously scavenge FA and ACR by forming various adducts, with the RV-ACR adduct (RA, molecular formula: C₁₇H₁₆O₄) and RV-ACR-FA adduct (RAF, molecular formula: C₁₈H₁₈O₅) being the dominant ones. The elimination of co-existing FA and ACR by RV were further confirmed in real food systems. RA (IC₅₀, 67.22 and 147.70 μM in GES-1 and Caco-2 cells, respectively) and RAF (127.50 and over 250 μM, respectively) showed significantly lower cytotoxicity than the co-existing FA and ACR (18.27 and 5.26 μM, respectively) in the gastrointestinal cell lines. This study provided data support for food safety control by employing RV as a dietary supplement to scavenge harmful aldehydes in foods.