Abstract
The O-alkyl cleavage enzyme is important for the metabolism of cytotoxic alkyl-lysophospholipids. We have developed a simple new method for the determination of the enzyme activity which is based on the formation of water-soluble phosphate during the enzyme reaction. This is the first assay which avoids the use of radiolabeled substrates.
MeSH terms
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Animals
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Hydrolases / metabolism*
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Lysophospholipids
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Microsomes, Liver / enzymology
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Phospholipase D / metabolism*
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Phospholipases / metabolism*
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Phospholipids / metabolism*
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Pteridines / metabolism*
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Rats
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Rats, Inbred Strains
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Type C Phospholipases / metabolism*
Substances
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Lysophospholipids
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Phospholipids
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Pteridines
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2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine
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Hydrolases
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Phospholipases
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Type C Phospholipases
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Phospholipase D
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O-alkyl cleavage enzyme