Synthesis of Spin-Labeled α-/β-Galactosylceramides and Glucosylceramides as Electron Paramagnetic Probes

J Org Chem. 2025 Jan 10;90(1):877-888. doi: 10.1021/acs.joc.4c02423. Epub 2024 Dec 16.

Abstract

α-/β-Galactosylceramide (GalCer) and glucosylceramide (GlcCer) derivatives having a radical label at the 6-C-position suitable for electron paramagnetic resonance spectroscopic studies were synthesized by a diversity-oriented strategy that is highlighted by the efficient glycosylation of a lipid precursor and late-stage ceramide assembly to enable lipid diversification. The strategy was also utilized to synthesize natural α-/β-GalCers and GlcCers. Furthermore, the involved azido-intermediates are flexible platforms to access various other GalCer and GlcCer derivatives.

MeSH terms

  • Electron Spin Resonance Spectroscopy
  • Galactosylceramides* / chemical synthesis
  • Galactosylceramides* / chemistry
  • Glucosylceramides* / chemical synthesis
  • Glucosylceramides* / chemistry
  • Molecular Structure
  • Spin Labels / chemical synthesis

Substances

  • Galactosylceramides
  • Glucosylceramides
  • Spin Labels