In-vitro Antioxidant, and In-vivo Analgesics and Anti-inflammatory Activity of Allamanda blanchetii Leaf Extract in Rats

Objective: This study assessed the In-vitro Antioxidant and In-vivo Analgesics and Anti-inflammatory Activity of Allamanda blanchetii Leaf Extract in Rats.

Introduction: Diverse pharmacological applications of plants from the Apocynaceae family are reported in the literature. Allamanda blanchetii; an ornamental species belonging to the Apocynaceae family, is characterized by diverse biological activities, i.e. antioxidant, cyto-toxic, thrombolytic, membrane-stabilizing, antimicrobial, and anti-proliferative effects. This species represents a perennial flora that thrives in tropical and subtropical climates.

Material and methods: Ultrasonication-assisted method used for plant extraction. The ex-tracts were subjected to phytochemical screening tests, followed by total phenolic content analysis, using gallic acid as a standard. The antioxidant activity was examined by DPPH scavenging and FRAP assays. The acetic acid-induced writhing test, tail flick, and Hot plate method were used for the determination of analgesic activity. Anti-inflammatory activity by following carrageenan-induced paw edema Results: ABLE treatments show analgesic effectiveness against the acid-induced pain source, tail flick, and hot plate methods at different doses of ABLE 400,200,100 mg/kg results showed respectively- 55.32, 38.67, and 22.85 (% inhibition), 89.47%, 62.57 %, 49.57%, and 100%, 92.40%, 65.33% response after 180 min of drug administration. ABLE 400 and 200 mg/kg exhibit effective results (1.43± 0.005 and 1.50± 0.008) against carra-geenan-induced intoxication.

Discussion: The fundamental components of antioxidants that can aid in the reduction of free radicals include phenol, flavonoids, and polyphenols. Applying DPPH and FRAP, ABLE exhibits remarkable antioxidant activity. When it comes to both centrally and periph-erally acting analgesics, ABLE exhibits highly effective activity. Methanolic ABLE had a noticeable impact on paw edema caused by carrageenan. Antioxidants, alkaloids, and gly-cosides were present in methanolic ABLE, which allowed it to efficiently combat inflam-matory mediators and the cause of pain.

Conclusion: Ultrasonic assistance is beneficial in isolating active metabolites in plants, with phenolic compounds exhibiting antioxidant activity. ABLE, a plant with 55% squalene, ef-fectively combats inflammatory mediators and pain. Further investigation is needed to iden-tify biomarkers in the plant.