19F-PCS measurements on proteins in live mammalian cells

Chem Commun (Camb). 2024 Dec 18. doi: 10.1039/d4cc05667a. Online ahead of print.

Abstract

Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. 19F-PCS is determined in live mammalian cells via dual labelling of the target protein with a paramagnetic tag and a 19F-tag, which is achieved by varied reactivity of solvent exposed cysteines in selection of different types of tags. About 0.1 ppm 19F-PCS can be observed over 20 Å from the paramagnetic center in the live cells.