Mitochondrial Membrane Potential and Lactate Dehydrogenase Activity in a Model of Acute In Vitro Ischemia/Reperfusion Injury

Deepthi Ashok; Haley Garbus; Obialunanma V Ebenebe

doi:10.1007/978-1-0716-4342-6_4

Mitochondrial Membrane Potential and Lactate Dehydrogenase Activity in a Model of Acute In Vitro Ischemia/Reperfusion Injury

Methods Mol Biol. 2025:2894:35-42. doi: 10.1007/978-1-0716-4342-6_4.

Authors

Deepthi Ashok¹, Haley Garbus², Obialunanma V Ebenebe³

Affiliations

¹ Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA.
² Bloomberg School of Public Health, Department of Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD, USA.
³ Bloomberg School of Public Health, Department of Environmental Health and Engineering, Johns Hopkins University, Baltimore, MD, USA. [email protected].

PMID: 39699808
DOI: 10.1007/978-1-0716-4342-6_4

Abstract

Experimental models of cardiac ischemia/reperfusion injury have served as useful tools in isolating the sequence of events and mechanisms involved following an infarct. The in vitro coverslip ischemia model in neonatal myocytes is key in observing acute cellular and organelle changes during ischemia and in reperfusion. Here we use neonatal mouse ventricular myocytes, and describe two experimental readouts of lactate dehydrogenase assay, for cell damage/injury and measurement of mitochondrial membrane potential.

Keywords: Acute ischemia/reperfusion; Cell culture; Mitochondria potential; Neonatal mouse ventricular myocytes.

MeSH terms

Animals
Animals, Newborn
Cells, Cultured
Disease Models, Animal
L-Lactate Dehydrogenase* / metabolism
Membrane Potential, Mitochondrial*
Mice
Myocardial Reperfusion Injury* / metabolism
Myocardial Reperfusion Injury* / pathology
Myocytes, Cardiac* / metabolism

Substances

L-Lactate Dehydrogenase