Cationic Liposome Formulation by Microfluidics for miRNA Delivery

Matthew Reily-Bell; Rishi Shah

doi:10.1007/978-1-0716-4342-6_14

Cationic Liposome Formulation by Microfluidics for miRNA Delivery

Methods Mol Biol. 2025:2894:163-172. doi: 10.1007/978-1-0716-4342-6_14.

Authors

Matthew Reily-Bell¹, Rishi Shah¹

Affiliation

¹ Department of Physiology and School of Pharmacy, Division of Health Sciences, University of Otago, Dunedin, New Zealand.

PMID: 39699818
DOI: 10.1007/978-1-0716-4342-6_14

Abstract

MiRNA therapeutics for treatment of cardiovascular disease face several problems with delivery. Encapsulation of miRNA therapeutics in cationic liposomes has shown potential to address many of these concerns with miRNA therapeutic delivery. Here we outline the formulation and characterization of cationic liposomes, capable of encapsulating and delivering miRNA therapeutics to AC16 cardiomyocyte cell lines, by microfluidics.

Keywords: Cationic; Liposomes; Microfluidics; Qubit; RNA protection assay; RT-qPCR; miRNA therapeutics.

MeSH terms

Cations* / chemistry
Cell Line
Gene Transfer Techniques
Humans
Liposomes* / chemistry
MicroRNAs* / genetics
Microfluidics* / instrumentation
Microfluidics* / methods
Myocytes, Cardiac / cytology
Myocytes, Cardiac / metabolism

Substances

Liposomes
MicroRNAs
Cations