Enhanced effect of ferrous sulfate on nitrogen retention and PBAT degradation during co-composting by combing with biochar-loaded FN1 bacterial composites

The treatment of biodegradable plastics through composting has garnered increasing attention. This study aimed to investigate the effects of Biochar FN1 bacteria and ferrous sulfate on nitrogen retention, greenhouse gas emissions, and degradable plastics during composting and to elucidate their synergistic mechanisms on microbial communities. Compared with the control, applying biochar-loaded FN1 bacteria composites combined with Ferrous sulfate (SGC) markedly accelerated organic matter degradation and reduced cumulative CO₂ and NH₃ emissions. The synergistic interaction between the composites and Ferrous sulfate significantly enhanced NH₄⁺-N levels in the thermophilic phase and NO₃^--N levels in the cooling phase, ultimately decreasing nitrogen loss by 14.9% (P < 0.05) and increasing the seed germination index (GI) by 22.5% (P < 0.05). Additionally, PBAT plastic degradation was improved by 31.6% (P < 0.05). The SGC treatment also altered the richness and diversity of the bacterial community in both the compost and the PBAT plastic sphere, particularly affecting Sphingobacterium, Pseudomonas, and Flavobacterium at the genus level. Symbiotic network analysis and Redundancy Analysis revealed that these functional degradation bacteria were significantly positively correlated with NO₃^--N levels and PBAT degradation. Furthermore, structural equation modelling indicated a positive relationship between PBAT degradation rate and composting temperature (r = 0.69, p < 0.05). The findings suggested that Fe²⁺ not only enhanced the FN1 activity but also promoted PBAT degradation by increasing ·OH content on the PBAT plastic sphere. Overall, the combined use of biochar-loaded FN1 bacteria and Ferrous sulfate effectively supports nitrogen retention and plastic degradation during composting.