Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants

Nobuo Arai; Yukino Tamamura-Andoh; Taketoshi Iwata; Ayako Watanabe-Yanai; Masahiro Kusumoto

doi:10.1016/j.mimet.2024.107082

Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants

J Microbiol Methods. 2025 Jan:228:107082. doi: 10.1016/j.mimet.2024.107082. Epub 2024 Dec 20.

Authors

Nobuo Arai¹, Yukino Tamamura-Andoh¹, Taketoshi Iwata¹, Ayako Watanabe-Yanai¹, Masahiro Kusumoto²

Affiliations

¹ Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
² Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka, Japan. Electronic address: [email protected].

PMID: 39710120
DOI: 10.1016/j.mimet.2024.107082

Abstract

We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.

Keywords: DNA chromatography; Multiplex allele-specific PCR; Salmonella 4,[5],12:I:-; Salmonella Typhimurium; Single-nucleotide polymorphisms; Subtyping.

MeSH terms

Alleles*
Chromatography / methods
DNA, Bacterial* / genetics
Genotype*
Genotyping Techniques / methods
Humans
Multiplex Polymerase Chain Reaction* / methods
Salmonella Infections / microbiology
Salmonella typhimurium* / classification
Salmonella typhimurium* / genetics
Salmonella typhimurium* / isolation & purification
Serogroup

Substances

DNA, Bacterial