The use of pesticides has significantly increased and proliferated following the technological advancements established by the green revolution, aimed at boosting agricultural productivity. The extensive use of man-made chemicals as fertilizer and pesticides has consequently led to large-scale application, which has led to a number of environmental and human health problems. This study has helped to develop a laser-induced graphene (LIG) sensor for the detection of the most widely used herbicide in the world, glyphosate. The electrochemical sensor developed is based on a three-dimensional porous and fibrous structure with nanosheets, making it suitable for scalable manufacture. The study was conducted utilising a linear voltammetry technique and demonstrates the potential to identify glyphosate with good sensitivity. The sensor exhibited detection and quantification limits of 2.7 μmol L-1 and 9.0 μmol L-1, respectively, and showed good selectivity without significant interference from other elements. The sensor presents advantages suitable for scalable production, with case studies in screening of glyphosate-contaminated samples.