California mice (Peromyscus californicus) adjust mouth movements for vocal production during early postnatal development

Background: The order Rodentia is the largest group of mammals. Diversification of vocal communication has contributed to rodent radiation and allowed them to occupy diverse habitats and adopt different social systems. The mechanism by which efficient vocal sounds, which carry over surprisingly large distances, are generated is incompletely understood. Here we focused on the development and function of rhythmic mouth movements and laryngeal sound production. We studied spontaneously vocalizing California mice (Peromyscus californicus) through video and sound recordings. Mouth gape was estimated from video images and vocal characteristics were measured in synchronized sound recordings.

Results: California mice coordinated their mouth movements with laryngeal sound production but differently in two call types. In high-frequency whistles ("USV syllables"), mouth movements were present on postnatal day 1 but were reduced within the first 2 weeks of life. Mouth movements were prominently present during sustained vocalizations ("SV syllables"), and movements became more and more adjusted to syllable beginning and end. Maximum mouth gape was correlated with sound intensity and fundamental frequency of SV syllables. The effect on sound intensity was the strongest during postnatal development and most predictable when the mouth was closed by temporarily immobilizing the mandible in an elevated position.

Conclusions: This study demonstrates that rhythmic orofacial behavior not only plays a critical role in determining acoustic features of the vocal behavior of California mice but also shows remarkable adjustments during early development.