Liquid-liquid separation in gut immunity

Front Immunol. 2024 Dec 10:15:1505123. doi: 10.3389/fimmu.2024.1505123. eCollection 2024.

Abstract

Gut immunity is essential for maintaining intestinal health. Recent studies have identified that intracellular liquid-liquid phase separation (LLPS) may play a significant role in regulating gut immunity, however, the underlying mechanisms remain unclear. LLPS refers to droplet condensates formed through intracellular molecular interactions, which are crucial for the formation of membraneless organelles and biomolecules. LLPS can contribute to the formation of tight junctions between intestinal epithelial cells and influence the colonization of probiotics in the intestine, thereby protecting the intestinal immune system by maintaining the integrity of the intestinal barrier and the stability of the microbiota. Additionally, LLPS can affect the microclusters on the plasma membrane of T cells, resulting in increased density and reduced mobility, which in turn influences T cell functionality. The occurrence of intracellular LLPS is intricately associated with the initiation and progression of gut immunity. This review introduces the mechanism of LLPS in gut immunity and analyzes future research directions and potential applications of this phenomenon.

Keywords: condensate; gut immunity; immune cell; inflammatory bowel disease; liquid-liquid separation.

Publication types

  • Review

MeSH terms

  • Animals
  • Gastrointestinal Microbiome* / immunology
  • Humans
  • Immunity, Mucosal
  • Intestinal Mucosa* / immunology
  • Intestinal Mucosa* / metabolism
  • Intestinal Mucosa* / microbiology
  • Probiotics
  • T-Lymphocytes / immunology
  • Tight Junctions / immunology
  • Tight Junctions / metabolism

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by Guangdong Basic and Applied Basic Research Foundation (Nos. 2021B1515420004), Guangdong Natural Science Foundation (SYPTKTA2021041), Natural Science Foundation of Guangdong Province (2020A1515011031), the program of Guangdong Provincial Clinical Research Center for Digestive Diseases (2020B1111170004), Guangdong Provincial Regional Joint Fund-Youth Fund Project, Grant/Award Number: 2021A1515110681, Science and Technology Projects in Guangzhou (202201020363).