ACK1/TNK2 kinase: molecular mechanisms and emerging cancer therapeutics

Trends Pharmacol Sci. 2025 Jan;46(1):62-77. doi: 10.1016/j.tips.2024.11.006. Epub 2024 Dec 24.

Abstract

Activated CDC42-associated kinase 1 (ACK1), encoded by the TNK2 gene, is a cytoplasmic non-receptor tyrosine kinase whose aberrant activation correlates positively with cancer severity. Recent research has revealed the functional relevance of this oncokinase - it is an epigenetic regulator that drives cancer progression in multiple malignancies. Although ACK1 is an attractive target for therapeutic intervention, incomplete knowledge of its diverse signaling mechanisms and the lack of specific inhibitors have challenged its clinical success. We summarize recent breakthroughs in understanding ACK1 regulation and cellular signaling, and shed light on its immunomodulatory role in balancing T cell activation. We provide a comprehensive overview of preclinical, proof-of-concept studies of potent ACK1-targeting small-molecule inhibitors that are expected to enter clinical trials for cancer patients.

Keywords: ACK1; T cells; TNK2; cancers; epigenetics; immune modulation; kinase inhibitors.

Publication types

  • Review

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use
  • Humans
  • Neoplasms* / drug therapy
  • Neoplasms* / enzymology
  • Protein Kinase Inhibitors / pharmacology
  • Protein Kinase Inhibitors / therapeutic use
  • Protein-Tyrosine Kinases* / antagonists & inhibitors
  • Protein-Tyrosine Kinases* / metabolism
  • Signal Transduction / drug effects

Substances

  • Protein-Tyrosine Kinases
  • TNK2 protein, human
  • Antineoplastic Agents
  • Protein Kinase Inhibitors