The Constituent-Dependent Translocation Mechanism for PM2.5 to Travel through the Olfactory Pathway

Sheng Wei; Ting Xu; Miao Cao; Huan Wang; Yiqun Song; Daqiang Yin

doi:10.1021/envhealth.4c00129

The Constituent-Dependent Translocation Mechanism for PM_2.5 to Travel through the Olfactory Pathway

Environ Health (Wash). 2024 Oct 1;2(12):856-864. doi: 10.1021/envhealth.4c00129. eCollection 2024 Dec 20.

Authors

Sheng Wei^{1

2}, Ting Xu^{1

3}, Miao Cao¹, Huan Wang¹, Yiqun Song¹, Daqiang Yin^{1

3}

Affiliations

¹ Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
² Postdoctoral Research Station of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
³ Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

Abstract

The neurotoxic risk of PM_2.5 is of worldwide concern, but the pathways through which PM_2.5 gets to the central nervous system are still under debate. The olfactory pathway provides a promising shortcut to the brain, which bypasses the blood-brain barrier for PM_2.5. However, direct evidence is lacking, and the translocation mechanism is still unclear. This study used the primary murine olfactory sensory neurons (OSNs) as an in vitro model to explore the translocation mechanism of PM_2.5 in the olfactory system. We found that PM_2.5 can be internalized into the OSNs via vesicle transportation. This process responds only to the water-insoluble compositions of PM_2.5 (WIS-PM_2.5) and cannot be affected by the water-soluble compositions of PM_2.5 (WS-PM_2.5). PM_2.5 can further disrupt the integrity of the barrier constituted by the OSNs, and WS-PM_2.5 plays a heightened role in inducing the damages. Our results suggested that both cellular and paracellular pathways are possibly involved in the translocation of PM_2.5 in the olfactory system. More advanced microscopy techniques need to be developed to explore the whole translocation process in the olfactory-brain pathway in both in vitro and in vivo models.