Detecting Hypoxia Through the Non-Invasive and Simultaneous Monitoring of Sweat Lactate and Tissue Oxygenation

Hypoxia, characterized by inadequate tissue oxygenation, may result in tissue damage and organ failure if not addressed. Current detection approaches frequently prove insufficient, depending on symptoms and rudimentary metrics such as tissue oxygenation, which fail to comprehensively identify the onset of hypoxia. The European Pressure Ulcer Advisory Panel (EPUAP) has recognized sweat lactate as a possible marker for the early identification of decubitus ulcers, nevertheless, neither sweat lactate nor oxygenation independently provides an appropriate diagnosis of hypoxia. We have fabricated a wearable device that non-invasively and concurrently monitors sweat lactate and tissue oxygenation to fill this gap. The apparatus comprises three essential components: (i) a hydrogel-based colorimetric lactate biosensor, (ii) a near-infrared (NIR) sensor for assessing tissue oxygenation, and (iii) an integrated form factor for enhanced wearability. The lactate sensor alters its hue upon interaction with lactate in sweat, whereas the NIR sensor monitors tissue oxygenation levels in real-time. The device underwent testing on phantom exhibiting tissue-mimicking characteristics and on human sweat post aerobic and anaerobic activities. Moreover, the device was demonstrated to be capable of real-time "on-body" simultaneous monitoring of sweat lactate spikes and tissue oxygenation (StO₂) drops, which showed strong correlation during a hypoxia protocol. This innovative technology has a wide range of potential applications, such as post-operative care, sepsis detection, and athletic performance monitoring, and may provide economical healthcare solutions in resource-limited regions.