Isolation and Characterization of Bioactive Compounds from Saccharomonospora sp. CMS18 and Their Antifungal Properties

Soohyun Um; Hyeongju Jeong; Ji-Eun Park; Jeongwon Seo; Sang Heon Jung; Munhyung Bae; Kyung-Tae Lee; Kyuho Moon

doi:10.3390/md22120539

Isolation and Characterization of Bioactive Compounds from Saccharomonospora sp. CMS18 and Their Antifungal Properties

Mar Drugs. 2024 Nov 30;22(12):539. doi: 10.3390/md22120539.

Authors

Soohyun Um¹, Hyeongju Jeong², Ji-Eun Park^{3

4}, Jeongwon Seo², Sang Heon Jung⁵, Munhyung Bae⁵, Kyung-Tae Lee⁴, Kyuho Moon^{6

7}

Affiliations

¹ College of Pharmacy, Yonsei University, Incheon 21983, Republic of Korea.
² Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea.
³ Korea Research Institute for Veterinary Biologics, Iksan 54531, Republic of Korea.
⁴ Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea.
⁵ College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea.
⁶ College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea.
⁷ Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.

Abstract

In this study, metagenomic analysis was employed to investigate the bacterial communities in the Muan tidal mudflat of the Republic of Korea. We used metagenomic analysis to identify the microbial community in tidal soil dominated by Proteobacteria. From this environment, the bacterial strain, Saccharomonospora sp. CMS18, was isolated and yielded two previously unknown compounds, penipaline D (3) and N-acetyl-dimethylallyltryptophan (4). The chemical structures of the isolated compounds along with 6-dimethylallyl-indole (1), 6-dimethylallyltryptophan (2), penipaline D (3), and N-acetyl-dimethylallyltryptophan (4) were structurally investigated using HR-ESI-MS and NMR spectroscopy. The isolated compound 6-dimethylallyl-indole (1) demonstrated broad-spectrum antifungal activity, with IC₅₀ value of 0.04 mM against Candida glabrata and 0.35 mM against both Candida albicans and Cryptococcus neoformans. Additionally, it exhibited additive interaction with caspofungin against C. albicans.

Keywords: antifungal agent; secondary metabolite; structural determination; tidal mudflat.

MeSH terms

Antifungal Agents* / chemistry
Antifungal Agents* / isolation & purification
Antifungal Agents* / pharmacology
Candida albicans* / drug effects
Candida glabrata* / drug effects
Caspofungin / pharmacology
Cryptococcus neoformans* / drug effects
Indoles / chemistry
Indoles / isolation & purification
Indoles / pharmacology
Magnetic Resonance Spectroscopy
Microbial Sensitivity Tests*
Republic of Korea
Saccharomycetales / drug effects

Substances

Antifungal Agents
Indoles
Caspofungin

Grants and funding

This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT) (2021R1C1C1009609 to K. Moon and 2022R1C1C2003274, 2021R1A6C101C369 to K.-T.L.; 2022R1A4A3022401 to K.-T.L. and K.M.) and the Korea Basic Science Institute under the R&D program (Project No. C230430 to K. Moon.) and supervised by the Ministry of Science and ICT. This research was partially supported by the “Regional Innovation Strategy (RIS)” through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (MOE) (2023-RIS-008 to K.-T.L.).