Deciphering the key role of biofilm and mechanisms in high-strength nitrogen removal within the anammox coupled partial S0-driven autotrophic denitrification system

Shuyan Yin; Yi-Xuan Wang; Cheng Hou; Jing Wang; Jing Xu; Xinbai Jiang; Dan Chen; Yang Mu; Jinyou Shen

doi:10.1016/j.biortech.2024.132020

Deciphering the key role of biofilm and mechanisms in high-strength nitrogen removal within the anammox coupled partial S⁰-driven autotrophic denitrification system

Bioresour Technol. 2024 Dec 26:419:132020. doi: 10.1016/j.biortech.2024.132020. Online ahead of print.

Authors

Shuyan Yin¹, Yi-Xuan Wang¹, Cheng Hou², Jing Wang³, Jing Xu³, Xinbai Jiang², Dan Chen³, Yang Mu⁴, Jinyou Shen⁵

Affiliations

¹ Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China; Engineering Research Centre of Chemical Pollution Control, Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
² Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
³ Engineering Research Centre of Chemical Pollution Control, Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
⁴ CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China.
⁵ Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Industry and Information Technology, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China; Engineering Research Centre of Chemical Pollution Control, Ministry of Education, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China. Electronic address: [email protected].

PMID: 39732373
DOI: 10.1016/j.biortech.2024.132020

Abstract

Anammox coupled partial S⁰-driven autotrophic denitrification (PS⁰AD) technology represents an innovative approach for removing nitrogen from wastewater. The research highlighted the crucial role of biofilm on sulfur particles in the nitrogen removal process. Further analysis revealed that sulfur-oxidizing bacteria (SOB) are primarily distributed in the inner layer of the biofilm, while anammox bacteria (AnAOB) are relatively evenly distributed in inner and outer layers, with Thiobacillus and Candidatus Brocadia being the dominant species, respectively. Except for anammox and PS⁰AD processes, ¹⁵N isotope labeling tests determined that sulfur reshaped nitrogen metabolism pathways, providing solid evidence for the occurrence of sulfammox process. SOB and AnAOB collaborate in nitrogen and sulfur conversion, with SOB-drived PS⁰AD processes reducing nitrate to nitrite for AnAOB to remove ammonia. Conversely, the nitrate produced from anammox process can be reused by SOB. Metagenomic analyses verified that SOB drove the PS⁰AD process through encoding soxBYZ gene, while AnAOB might play an important role in simultaneously driving the anammox and sulfammox processes. These findings underscore the importance of biofilm and clarify the nitrogen-sulfur cycle mechanisms within the coupled system.

Keywords: Anammox; Biofilm; Microbial interactions; Nitrogen-sulfur cycle; Partial S(0)-driven autotrophic denitrification.