Proteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions

Rafael Eduardo Hernández-Guisao; Ricardo Axayacatl González-García; Tim McCubbin; Pamela Guerra-Blanco; Edgar Salgado Manjarrez; Juan Aranda-Barradas; Antonio Velasco; Elvia Inés García-Peña

doi:10.1016/j.biortech.2024.132023

Proteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions

Bioresour Technol. 2024 Dec 26:419:132023. doi: 10.1016/j.biortech.2024.132023. Online ahead of print.

Authors

Rafael Eduardo Hernández-Guisao¹, Ricardo Axayacatl González-García², Tim McCubbin³, Pamela Guerra-Blanco⁴, Edgar Salgado Manjarrez⁵, Juan Aranda-Barradas⁵, Antonio Velasco¹, Elvia Inés García-Peña⁶

Affiliations

¹ Bioprocesses Department, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico.
² Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Brisbane 4072, Australia; Integrated Design Environment for Advanced Biomanufacturing (IDEA Bio), The University of Queensland, Queensland, Brisbane 4072, Australia.
³ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Brisbane 4072, Australia; ARC Centre of Excellence in Synthetic Biology, The University of Queensland, Queensland, Australia.
⁴ Escuela Superior de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional, P.O. Box 07340, México City, México.
⁵ Bioengineering Department Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico.
⁶ Bioprocesses Department, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico. Electronic address: [email protected].

PMID: 39732376
DOI: 10.1016/j.biortech.2024.132023

Abstract

A biohydrogen and polyhydroxyalkanoates (PHA)-producing natural photoheterotrophic mixed culture composed mainly by Rhodopseudomonas palustris and Clostridium sp was studied by a proteomic analysis under non-growth conditions (nitrogen-absence and organic acids). Proteins in C. pasteurianum were upregulated, particularly those related to stress response. In contrast, C. pasteurianum in the consortium did not present such proteins, showing the advantage of being part of it. Both cultures showed proteins involved in organic acid metabolism and biohydrogen production, such as lactate dehydrogenase, ferredoxins, and hydrogenases. Proteomes of R. palustris as single culture and in consortium showed that organic acids were redirected into central carbon pathways to generate reduced equivalents for biohydrogen production. Light-harvesting proteins and fatty acid metabolism linked to PHA accumulation were also upregulated. This study provides insights into how the proteomes of individual organisms and their consortium counterparts adapt to non-growth conditions, shedding light on how microbial interactions influence protein expression.

Keywords: Clostridium sp.; Microbial consortia; Organic acids; Photofermentation; Proteins expression; Rhodopseudomonas palustris; bioH(2).