Hybrids of copper cysteamine nanosheets and silver nanocluster ensure superior bactericidal capability via enhanced photodynamic effect

Sci Rep. 2024 Dec 28;14(1):31273. doi: 10.1038/s41598-024-82738-4.

Abstract

Over the past decades, bacterial infections resulting from the misuse of antibiotics have garnered significant attention. Among the alternative antibacterial strategies, photodynamic therapy (PDT) has emerged as a promising non-antibiotic approach. However, persistent bacterial biofilms, particularly those composed of gram-negative bacteria with their protective outer membranes, have exhibited remarkable resilience to PDT. To address this challenge, we have developed an antibacterial composite material, silver nanoparticles coated-copper cysteamine (Cu-Cy-PEG@AgNPs), which demonstrates remarkable antibacterial activity against both gram-positive and gram-negative bacteria. Specifically, under UV irradiation, Cu-Cy-PEG@AgNPs achieves a sterilization efficiency of approximately 100% at a low concentration of 25 µg/mL. The incorporation of silver nanoparticles significantly enhances the antibacterial performance of Cu-Cy, effectively eradicating persistent bacterial biofilm infections. Moreover, Cu-Cy-PEG@AgNPs exhibits excellent biocompatibility with L929 cells, indicating its potential for use in relevant applications to combat bacterial infections.

Keywords: Antibacterial activity; Copper cysteamine; Reactive oxygen species (ROS); Silver nanoparticles.

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Biofilms* / drug effects
  • Cell Line
  • Copper* / chemistry
  • Copper* / pharmacology
  • Cysteamine* / chemistry
  • Cysteamine* / pharmacology
  • Metal Nanoparticles* / chemistry
  • Mice
  • Microbial Sensitivity Tests
  • Photochemotherapy* / methods
  • Silver* / chemistry
  • Silver* / pharmacology

Substances

  • Silver
  • Copper
  • Anti-Bacterial Agents
  • Cysteamine