A PRA-Rab trafficking machinery modulates NLR immune receptor plasma membrane microdomain anchoring and blast resistance in rice

Di Liang; Dongyong Yang; Tai Li; Zhe Zhu; Bingxiao Yan; Yang He; Xiaoyuan Li; Keran Zhai; Jiyun Liu; Yoji Kawano; Yiwen Deng; Xu Na Wu; Junzhong Liu; Zuhua He

doi:10.1016/j.scib.2024.12.007

A PRA-Rab trafficking machinery modulates NLR immune receptor plasma membrane microdomain anchoring and blast resistance in rice

Sci Bull (Beijing). 2024 Dec 9:S2095-9273(24)00911-3. doi: 10.1016/j.scib.2024.12.007. Online ahead of print.

Authors

Di Liang¹, Dongyong Yang², Tai Li³, Zhe Zhu³, Bingxiao Yan², Yang He², Xiaoyuan Li⁴, Keran Zhai², Jiyun Liu², Yoji Kawano⁵, Yiwen Deng⁶, Xu Na Wu³, Junzhong Liu⁷, Zuhua He⁸

Affiliations

¹ CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
² CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
³ Yunnan Key Laboratory of Cell Metabolism and Diseases, State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan, Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650500, China.
⁴ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁵ Institute of Plant Science and Resources, Okayama University, Okayama 710-0046, Japan.
⁶ CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
⁷ Yunnan Key Laboratory of Cell Metabolism and Diseases, State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan, Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming 650500, China. Electronic address: [email protected].
⁸ CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100039, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: [email protected].

PMID: 39736493
DOI: 10.1016/j.scib.2024.12.007

Abstract

Nucleotide-binding leucine-rich repeat (NLR) receptors mediate pathogen effector-triggered immunity (ETI) in plants, and a subclass of NLRs are hypothesized to function at the plasma membrane (PM). However, how NLR traffic and PM delivery are regulated during immune responses remains largely unknown. The rice NLR PigmR confers broad-spectrum resistance to the blast fungus Magnaporthe oryzae. Here, we report that a PRA (Prenylated Rab acceptor) protein, PIBP4 (PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 4), interacts with both PigmR and the active form of the Rab GTPase, OsRab5a, thereby loads a portion of PigmR on trafficking vesicles that target to PM microdomains. Microdomain-localized PigmR interacts with and activates the small GTPase OsRac1, which triggers reactive oxygen species signaling and hypersensitive response, leading to immune responses against blast infection. Thus, our study discovers a previously unknown mechanism that deploys a PRA-Rab protein delivering hub to ensure ETI, linking the membrane trafficking machinery with NLR function and immune activation in plants.

Keywords: Blast resistance; OsRab5a; PigmR; Prenylated Rab acceptor; Trafficking vesicles.