Brainstem BDNF neurons are downstream of GFRAL/GLP1R signalling

Nat Commun. 2024 Dec 30;15(1):10749. doi: 10.1038/s41467-024-54367-y.

Abstract

Growth differentiation factor 15, GDF15, and glucagon-like peptide-1 (GLP-1) analogues act through brainstem neurons that co-localise their receptors, GDNF-family receptor α-like (GFRAL) and GLP1R, to reduce food intake and body weight. However, their use as clinical treatments is partially hampered since both can also induce sickness-like behaviours, including aversion, that are mediated through a well-characterised pathway via the exterolateral parabrachial nucleus. Here, in mice, we describe a separate pathway downstream of GFRAL/GLP1R neurons that involves a distinct population of brain-derived neurotrophic factor (BDNF) cells in the medial nucleus of the tractus solitarius. Thus, BDNFmNTS neurons are required for the weight-reducing actions of both GDF15 and the GLP1RA, Exendin-4. Moreover, acute activation of BDNFmNTS neurons is sufficient to reduce food intake and drive fatty acid oxidation and might provide a route for longer-term weight loss.

MeSH terms

  • Animals
  • Body Weight
  • Brain Stem* / metabolism
  • Brain-Derived Neurotrophic Factor* / metabolism
  • Eating / physiology
  • Exenatide / pharmacology
  • Glial Cell Line-Derived Neurotrophic Factor Receptors* / genetics
  • Glial Cell Line-Derived Neurotrophic Factor Receptors* / metabolism
  • Glucagon-Like Peptide 1 / metabolism
  • Glucagon-Like Peptide-1 Receptor* / metabolism
  • Growth Differentiation Factor 15* / genetics
  • Growth Differentiation Factor 15* / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neurons* / metabolism
  • Signal Transduction*
  • Solitary Nucleus / metabolism

Substances

  • Brain-Derived Neurotrophic Factor
  • Glucagon-Like Peptide-1 Receptor
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • Glp1r protein, mouse
  • Growth Differentiation Factor 15
  • Bdnf protein, mouse
  • Exenatide
  • Glucagon-Like Peptide 1