Anti-CTLA4 treatment reduces lymphedema risk potentially through a systemic expansion of the FOXP3+ Treg population

Nat Commun. 2024 Dec 30;15(1):10784. doi: 10.1038/s41467-024-55002-6.

Abstract

Secondary lymphedema is a common sequel of oncologic surgery and presents a global health burden still lacking pharmacological treatment. The infiltration of the lymphedematous extremities with CD4+T cells influences lymphedema onset and emerges as a promising therapy target. Here, we show that the modulation of CD4+FOXP3+CD25+regulatory T (Treg) cells upon anti-CTLA4 treatment protects against lymphedema development in patients with melanoma and in a mouse lymphedema model. A retrospective evaluation of a melanoma patient registry reveals that anti-CTLA4 reduces lymphedema risk; in parallel, anti-CTLA4 reduces edema and improves lymphatic function in a mouse-tail lymphedema model. This protective effect of anti-CTLA4 correlates with a systemic expansion of Tregs, both in the animal model and in patients with melanoma. Our data thus show that anti-CTLA4 with its lymphedema-protective and anti-tumor properties is a promising candidate for more diverse application in the clinics.

MeSH terms

  • Animals
  • CTLA-4 Antigen* / antagonists & inhibitors
  • Disease Models, Animal
  • Female
  • Forkhead Transcription Factors* / metabolism
  • Humans
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Lymphedema* / etiology
  • Lymphedema* / immunology
  • Lymphedema* / prevention & control
  • Male
  • Melanoma* / drug therapy
  • Melanoma* / immunology
  • Mice
  • Mice, Inbred C57BL
  • Middle Aged
  • Retrospective Studies
  • T-Lymphocytes, Regulatory* / drug effects
  • T-Lymphocytes, Regulatory* / immunology

Substances

  • Forkhead Transcription Factors
  • CTLA-4 Antigen
  • FOXP3 protein, human
  • CTLA4 protein, human
  • Foxp3 protein, mouse
  • Interleukin-2 Receptor alpha Subunit