Expression of Wnt signaling proteins LEF1, β-catenin, GSK3β, DVL1, and N-myc varies across retinoblastoma subtypes and pRb phosphorylation status

Sci Rep. 2024 Dec 30;14(1):31725. doi: 10.1038/s41598-024-82044-z.

Abstract

Retinoblastoma, a rare childhood eye cancer, has hereditary and non-hereditary forms. While TNM classification helps in prognosis, understanding molecular mechanisms is vital for the clinical behavior of retinoblastoma prediction. Our study aimed to analyze the expression levels of key Wnt pathway proteins, GSK3β, LEF1, β-catenin, and DVL1, and associate them to non-phosphorylated active form (pRb) and the phosphorylated inactive form (ppRb) and N-myc expression, in retinoblastoma cells and healthy retinal cells, in order to elucidate their roles in retinoblastoma and identify potential targets that could help to improve diagnostic and therapy. Specimens from 22 retinoblastoma cases (unilateral, bilateral, and trilateral) were analyzed. Immunohistochemistry assessed proteins' expressions, followed by semi-quantitative analysis using the Immunoreactivity Score (IRS). Bayesian statistical methods were employed for data analysis. The study revealed various expression patterns of Wnt signaling proteins across different retinoblastoma types. The high expression levels were observed for LEF1 and DVL1. Inactive GSK3β and nuclear localization of β-catenin indicated Wnt signaling activation. The levels of inactive ppRb were significantly higher in retinoblastoma compared to healthy retina, as well as the levels of inactive GSK3β. Positive correlations between DVL1 and N-myc, GSK3β Y216 and GSK3β S9 and non-P β-catenin and LEF1 were established. Retinoblastomas without germline mutations (RB1+/+) exhibited high pRb, N-myc, and LEF1 levels, while those in genetically predisposed children (RB1+/-) showed lower expression of these proteins. Trilateral retinoblastomas demonstrated especially high N-myc and LEF1, but low pRb and ppRb levels. The findings highlight the meaningful role of the Wnt signaling pathway in retinoblastoma pathogenesis, providing insights into potential therapeutic targets. Understanding molecular features may pave the way for personalized treatments and improve outcomes for retinoblastoma patients.

Keywords: RB1; DVL1; GSK3β; LEF1; N-myc; Retinoblastoma; Wnt signaling pathway; pRb; ppRb; β-catenin.

MeSH terms

  • Child
  • Child, Preschool
  • Dishevelled Proteins* / genetics
  • Dishevelled Proteins* / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic
  • Glycogen Synthase Kinase 3 beta* / genetics
  • Glycogen Synthase Kinase 3 beta* / metabolism
  • Humans
  • Infant
  • Lymphoid Enhancer-Binding Factor 1* / genetics
  • Lymphoid Enhancer-Binding Factor 1* / metabolism
  • Male
  • Phosphorylation
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism
  • Retinal Neoplasms / genetics
  • Retinal Neoplasms / metabolism
  • Retinal Neoplasms / pathology
  • Retinoblastoma Protein / genetics
  • Retinoblastoma Protein / metabolism
  • Retinoblastoma* / genetics
  • Retinoblastoma* / metabolism
  • Retinoblastoma* / pathology
  • Wnt Signaling Pathway*
  • beta Catenin* / genetics
  • beta Catenin* / metabolism

Substances

  • Lymphoid Enhancer-Binding Factor 1
  • Glycogen Synthase Kinase 3 beta
  • Dishevelled Proteins
  • beta Catenin
  • LEF1 protein, human
  • DVL1 protein, human
  • Retinoblastoma Protein
  • GSK3B protein, human
  • Proto-Oncogene Proteins c-myc
  • CTNNB1 protein, human
  • MYC protein, human