Health Benefits of Monk Fruit under Traditional Dietary Patterns: Perspective on Immunity and Gut Microbiota Modulatory Functions

Monk fruit is the mature fruit of Siraitia grosvenorii (Swingle) C. Jeffrey (SG), which contains mogrosides and various nutrients with diverse benefits as a traditional edible herb. The immunomodulatory effects of the ingredients of monk fruit in daily diets are poorly understood. Monk fruit juice concentrate is a commercial product of monk fruit and in this study, the immune-enhancing activity, immunosuppressive prevention, and gut microbiota modulatory effects of the long-term consumption of its diluent (called SG juice) in daily drinking were investigated in both healthy and cyclophosphamide (CTX)-treated mice. The results indicated that SG juice consumption was beneficial to weight management and improved immunity in mice by enhancing various immune factors. 16S rRNA analysis found that SG juice impacted the diversity and gut microbiota composition with the enrichment of immune-related flora, including Alloprevotella, Bifidobacterium_pseudolongum, Lactobacillus, and Bacteroides_sartorii. Additionally, the daily intake of SG juice exerted preventive effects on immunosuppressive mice with the recovery of reduced body weight and immunoglobulin levels, and restoration of gut microbiota imbalance. These data provide scientific insights into the immunomodulatory effects of monk fruit and foresee its application in functional foods.