Globally the incidence and prevalence of chronic kidney disease (CKD) is increasing day by day. Various biochemical and hematological abnormalities are associated with CKD that leads to morbidity and mortality. The aim of this observational study was to evaluate the socio-demographic profile, biochemical and hematological parameters of chronic kidney disease patients in Mymensingh Medical College Hospital, Mymensingh, Bangladesh from January 2020 to December 2020. This study also showed variation of biochemical parameters with lower calcium and hemoglobin level which was due to improper renal function. A total of 179 End Stage Renal Disease (ESRD) patients were enrolled in this study. Male and female ratio was 2.086:1. This study had more male patients than female. The Mean±SD age of the patient was 46.14±14.25 years and maximum of the CKD patients belonged to the age group of 41-60 years. This study depicted Glomerulonephritis was the leading cause of CKD. Second common cause was Diabetes mellitus. A portion of CKD was unknown etiology (CKDu) which was found 17.31%. Routine biochemical parameters were also seen in this study. This study showed that the majority of the patients were living below the level of poverty and came from rural area. This study aimed to evaluate the derangements of serum biochemical parameter and the association of risk factors with CKD. These demographic character, biochemical profile and etiology have association with CKD progression. So, early evaluation of these parameters may reduce the incidence of CKD.