Helicobacter pylori (H pylori) infection is known to be associated with dyspepsia for more than three decades. This study was conducted to investigate the frequency of H pylori infection in functional dyspepsia. Helicobacter pylori infection was detected by stool antigen tests were analyzed in 102 patients with functional dyspepsia. The functional dyspepsia was diagnosed according to Rome IV criteria as well as normal upper GI (Gastro-intestinal) endoscopy and abdominal ultrasonography within six months. In this study, H pylori infection rate diagnosed by the stool antigen test was detected to be 26.5% among the people with functional dyspepsia. Sex-based proportion H pylori infection was 21.2% for men and 29.0% for women with functional dyspepsia. The highest prevalent group with Helicobacter pylori infection was 30-39 years. In this study, 25.75% of rural participants and 27.78% of urban participants with functional dyspepsia were positive for H pylori. The stool antigen positive group and the stool antigen negative group were not significantly different in respect to age (p=0.29), sex (p=0.41) and residence (p=0.82). This study showed an increased stool antigen positivity rate for H pylori infection among the patients with functional dyspepsia. Further studies are required to determine the exact prevalence of H pylori among this group of patients.