Blood stream infection (BSI) is of the most devastating infection of any hospital which has a high mortality. Staphylococcus aureus, coagulase-negative staphylococci (CONS) and enterococcus species are commonest isolated bacteria. This was a cross-sectional descriptive study conducted at the Sylhet Women's Medical College Hospital from October 2022 to March 2023. Total study period was 6 months. Data were collected from electronic medical record (EMR). Out of a total 1674 blood samples 315 samples were positive for blood culture (18.8%). The mean age of the participants was 15.6 year with a highest frequency in the below 20-year age group. Staphylococcus aureus was commonest isolated bacteria followed by CONS. Imipenem (89.0%), Piperacillin and Tazobactam (88.0%), Doxycycline (81.0%) and Amikacin (79.0%) were most sensitive antibiotics. Precise bacteriological profile and antibiogram is helpful for any institution to have an idea about the current infection pattern. Commonly used empirical antibiotics should be selected according to antibiogram of that particular hospital.