Development of a multifunctional and sustainable pterostilbene nanoemulsion incorporated chitosan-alginate food packaging film for shiitake mushroom preservation

The pervasive use of petroleum-based food packaging has caused significant ecological damage due to their unsustainability and non-biodegradability. Polysaccharide-based biodegradable materials are promising alternatives, but low hydrophobicity and functional properties limit their practical applications which can be overcome by incorporation of phytochemical(s). Therefore, by leveraging the strong antioxidant and antibacterial potential of pterostilbene (PTB), we have developed PTB nanoemulsion (NE) incorporated chitosan/sodium alginate (CS/SA) film for food packaging applications. The PTBNE was prepared by high pressure homogenization and characterized for particle size distribution and morphology via DLS, TEM and AFM. The PTBNE CS/SA film was developed by solvent casting method and demonstrated improved mechanical, optical, water resistance and oxygen barrier properties as compared to native CS/SA film. The films were characterized via SEM, 3D optical profilometry, FTIR, XRD and TGA analysis to assess morphological and structural variations. Notably, incorporation of PTBNE in CS/SA matrix significantly enhanced the antioxidant and antibacterial potential of film along with biocompatibility in fibroblast cells. The developed PTBNE CS/SA film demonstrated comparable results with polythene in post harvested shiitake mushroom preservation up to 10 days with rapid soil degradation. Overall, the findings suggested that PTBNE CS/SA film can be a promising alternative to conventional petroleum-based packaging materials.